Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [hiplex3]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: HiPlex BreakTag dataset targeting 300 sites with SNPs targeting the reference and mutant allele (HiPlex3)