Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation after 24 hrs cells were infected with mycobacterium tuberculosis to identify the host factors involved in infection. Total RNA was colloected from bmdm cells stimulated with cytokine and /or infected with mycobacterium tuberculosis in a time course experiments from 28hrs to 72 hrs.