Percentage of plant taxa amplified in silico.
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From zero to three nucleotide mismatches between each target primer and database sequences were allowed in ecoPCR, except for the two bases at the 3′-end of the primer.*ITS2, which was used for the construction of subset 1, was used as a reverse primer in ecoPCR.†ITS5, which was used for the construction of subset 2, was used as a forward primer in ecoPCR.‡ITS4, which was used for the construction of subset 2, was used as a reverse primer in ecoPCR.§ITS3, which was used for the construction of subset 3, was used as a forward primer in ecoPCR.
创建时间：
2015-12-02
