Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del:+-Del/+ - 5194_5231

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration &gt; 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy &lt; 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.本研究对成对陌生个体间的社交互动与交流行为开展了评估。针对本次社交行为与超声发声(USVs)录制工作，鉴于当前动物近距离互动时无法识别超声发声的发声个体，因此研究聚焦于成对个体的相关数据采集。据此，我们对来自不同饲养笼、年龄一致（14至20周龄）且基因型相同的两只陌生个体，进行了为期47小时（两个昼夜）的无干扰成对社交互动录制。为完成该录制工作，我们将活体小鼠追踪器(Live Mouse Tracker)系统（插件931）与1台CM16/CMPA型麦克风（德国格利尼克Avisoft Bioacoustics公司出品）相连接，并将该麦克风接入Avisoft Ultrasound Gate 416设备，其参数设置如下：采样率300 kHz，16位格式；触发条件：该通道电平；预触发时长1 s；保持时长1 s；有效时长>0.005 s；触发事件：25-125 kHz频段内能量占比2%且熵值<50%（设备及参数均由德国格利尼克Avisoft Bioacoustics公司提供）。LMT系统与Avisoft系统依据de Chaumont等（2021）发表的实验方案完成同步校准。每份上传内容均包含经处理且完成超声发声同步的LMT sqlite数据库，以及由Avisoft自动录制的全部超声发声序列压缩包（zip格式）。