Saccharification Index Data for MyDFF Study - Supplementary Figure S5

This dataset contains Saccharification Index (SI) values from a randomized crossover dietary fiber intervention trial. The data supports Figure S5 in the supplementary materials of the manuscript titled "A personalized microbiota screening platform for optimizing individual volatile fatty acid production - A proof-of-concept study", submitted to the journal Microbiome. Study Design:- Randomized crossover trial with 8 healthy Japanese adults (aged 34-62 years)- Three phases per participant: Baseline (weeks 1-3), L-PDF intervention (weeks 4-6 or 7-9), M-PDF intervention (weeks 7-9 or 4-6)- Daily supplementation with 6g personalized dietary fiber during intervention phases- 9 fecal samples per participant (3 per phase) = 72 total longitudinal samples Data Collection:SI values were measured from in vitro fermentation of fecal microbiota samples collected across three study phases. Participants 1-3 were screened with 17 dietary fiber products; participants 4-8 were screened with 13 products (4 products were discontinued during the study). Each participant received personalized Most Prebiotic Dietary Fiber (M-PDF) and Least Prebiotic Dietary Fiber (L-PDF) based on individual MyDFF screening results. Each SI measurement represents the mean of triplicate fermentation experiments and is calculated as:SI = (pH_control - pH_sample) / (pH_control - pH_glucose) Files Included:- FigS5_Intervention_SI_Data.csv: Plain text CSV file with all SI measurements (72 samples × 17 fiber products)- FigS5_Intervention_SI_Data.xlsx: Excel workbook with formatted data and detailed notes- README.md: Comprehensive documentation of the intervention study and dataset Study Registration: Japan Registry of Clinical Trials (jRCTs051220163)本数据集包含来自一项随机交叉膳食纤维干预试验的糖化指数（Saccharification Index, SI）值。该数据支撑了投稿于期刊《Microbiome》、题为《用于优化个体挥发性脂肪酸生成的个性化菌群筛选平台——一项概念验证研究》的手稿补充材料中的图S5。 ### 研究设计 - 纳入8名健康日本成年人（年龄34~62岁）开展随机交叉试验 - 每名受试者设置三个阶段：基线期（第1~3周）、L-PDF（Least Prebiotic Dietary Fiber, 最差益生元膳食纤维）干预期（第4~6周或第7~9周）、M-PDF（Most Prebiotic Dietary Fiber, 最优益生元膳食纤维）干预期（第7~9周或第4~6周） - 干预阶段每日补充6g个性化膳食纤维 - 每名受试者采集9份粪便样本（每阶段3份），共计72份纵向样本 ### 数据采集 SI值通过对三个研究阶段采集的粪便菌群样本进行体外发酵实验测得。其中，受试者1~3使用17种膳食纤维产品进行筛选，受试者4~8使用13种膳食纤维产品进行筛选（研究期间有4种产品停产）。每名受试者根据个人MyDFF筛选结果，分别接受L-PDF与M-PDF干预。 每份SI测量值为三次重复发酵实验的均值，计算公式为： SI = (pH_对照 - pH_样本) / (pH_对照 - pH_葡萄糖) ### 包含文件 - FigS5_Intervention_SI_Data.csv：纯文本CSV格式文件，收录全部SI测量值（72份样本 × 17种膳食纤维产品） - FigS5_Intervention_SI_Data.xlsx：包含格式化数据与详细注释的Excel工作簿 - README.md：涵盖本干预研究与数据集的完整说明文档 ### 研究注册 本研究在日本临床试验注册中心（Japan Registry of Clinical Trials, jRCTs051220163）完成注册。