Methodological Guide on Mesotherapy and Biorevitalization Within the Prejuvenation Paradigm: A Comprehensive Analysis of Efficacy and Clinical Protocols for Young Facial Skin

This study investigates the clinical application of injectable aesthetic modalities—mesotherapy and biorevitalization—in patients aged 18–35 years. The analysis is based on a systematic review of specialized literature, clinical observations, and market data from 2023–2024. The conceptual framework of the study is grounded in the paradigm shift from corrective aesthetic dermatology toward a preventive model of care, defined as prejuvenation. This approach emphasizes early intervention aimed at preserving the structural integrity and functional capacity of the skin and minimizing the development of clinically visible signs of aging. The paper explores the molecular and biological mechanisms of action of polynucleotides (PDRN/PN), amino acid complexes, and hyaluronic acid, with consideration of the physiological characteristics of young facial skin. Particular attention is given to their effects on dermal matrix metabolism, reparative processes, and tissue hydration regulation at preclinical stages of skin aging. The comparative analysis section evaluates different product classes in relation to their clinical efficacy across common aesthetic indications in this age group. Outcome assessment is based on correlating clinical endpoints with objective parameters, enabling evidence-informed selection of formulations and injection techniques according to dominant pathogenetic mechanisms.本研究针对18至35岁人群，探讨注射美容手段——中胚层疗法（mesotherapy）与生物焕肤疗法（biorevitalization）的临床应用。本分析基于2023至2024年的专业文献系统综述、临床观察与市场数据。 本研究的概念框架基于美容皮肤科从修复型向预防性护理模式的范式转变，该模式被定义为预年轻化（prejuvenation）。此方案强调早期干预，旨在维持皮肤结构完整性与功能活性，并减少临床可见衰老征象的发生发展。 本研究探讨了多核苷酸（PDRN/PN）、氨基酸复合物及透明质酸的分子与生物学作用机制，并结合年轻面部皮肤的生理特征展开分析。研究重点关注上述成分在皮肤衰老临床前阶段，对真皮基质代谢、修复过程及组织水合调节的作用效果。 本研究的对比分析部分，针对该年龄段人群常见的美容适应证，评估不同产品类别的临床疗效。结局评估通过将临床终点指标与客观参数相关联，从而能够依据主要致病机制，实现对制剂配方与注射技术的循证选择。