LFY is pioneer transcription factor [MNase-seq]

This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor. MNase seq of 35S:LFY-GR root explants. Tissues were treated with dexamethasone (after LFY binding) or mock (before LFY binding) solution. Two MNase concentrations were used: low MNase treatment, which preserve fragile nucleosomes and standard (high) MNase treatment, in which fragile nucleosomes are lost. Two biological replicates were sequenced for each condition.