High throughput phenotypic screen and transcriptional analysis identify new compounds, targets and pathways for macrophage reprogramming II

Purpose: To understand compound effects on human macrophage repolarization in the transcriptional level Overall design: Fresh isolated human blood monocytes were cultured with 50ng/mL M-CSF for 7 days to generate M0 status macrohages. Macrophages were differentiated into M1 macrophages (M1 Mac) by 50ng/mL IFNg plus 50ng/mL TNFa or M2 macrophages (M2 Mac) by 10ng/mL IL4 plus 10ng/mL IL13. M2 Mac were treated with different M1-type compounds or IFNg or DMSO for 24 hrs. M1 Mac were treated with different M2-type compounds or IL4 or DMSO for 24 hrs. RNA was isolated and purified by Qiagen Rneasy micro kit and applied to RNAseq.