Dataset for: Nematode‐associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats

Marine nematode microbiomes were studied by isolating host-associated microbial taxa in nematodes from the Gulf of Mexico. A two‐gene metabarcoding approach was used to amplify the V4 region of the 16S ribosomal RNA (rRNA) gene targeting bacteria/archaea and the V1–V2 region of the 18S rRNA gene targeting microbial eukaryotes. This dataset contains nematode taxonomic identifications, video images, and 18S rRNA, 16S rRNA, NGS reads (fastq) from samples collected in the Gulf of Mexico in June and July of 2007. This dataset supports the publications: Schuelke, T., Pereira, T.J., Hardy, S.M., & Bik, H.M. (2018). Nematode-associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats. Molecular Ecology, 27(8): 1930-1951. doi: 10.1111/mec.14539; and Pereira, T. J., De Santiago, A., Schuelke, T., Hardy, S. M., & Bik, H. M. (2020). The impact of intragenomic rRNA variation on metabarcoding‐derived diversity estimates: A case study from marine nematodes. Environmental DNA. doi:10.1002/edn3.77

本数据集以墨西哥湾采集的线虫为研究材料，通过分离其宿主关联微生物类群，对海洋线虫菌群进行系统探究。研究采用双基因元条形码（metabarcoding）技术：针对细菌与古菌，扩增16S核糖体RNA（16S rRNA）基因的V4区；针对真核微生物，扩增18S核糖体RNA（18S rRNA）基因的V1–V2区。本数据集包含2007年6月至7月于墨西哥湾采集样本的线虫分类鉴定结果、视频影像，以及18S rRNA、16S rRNA相关二代测序（NGS）读段（fastq格式文件）。本数据集支持以下两篇已发表学术论文：Schuelke, T.、Pereira, T.J.、Hardy, S.M. 与 Bik, H.M.（2018）。《海洋沉积物生境中线虫相关微生物类群与宿主系统发育、地理区域或取食形态无关联》，《分子生态学》（*Molecular Ecology*），27(8): 1930-1951. doi: 10.1111/mec.14539；以及Pereira, T. J.、De Santiago, A.、Schuelke, T.、Hardy, S. M. 与 Bik, H. M.（2020）。《基因组内rRNA变异对元条形码衍生多样性估算的影响：以海洋线虫为例》，《环境DNA》（*Environmental DNA*），doi:10.1002/edn3.77