Structural characterization and antifungal activity of Algerian Opuntia ficus-indica polysaccharides: FTIR, GC-MS, colorimetric assays, and bioactivity dataStructural and antifungal data: Sequentially extracted polysaccharides from Algerian Opuntia ficus-indica cladodes (FTIR, GC, colorimetric assays)

This dataset comprises raw and processed Fourier-transform infrared (FTIR) spectra obtained via attenuated total reflectance (ATR) for six polysaccharide fractions extracted from Algerian Opuntia ficus-indica cladodes: Mucilage 1 & 2 (water-soluble fractions) Pectin 1 & 2 (hot water/EDTA-extracted) Hemicellulose (alkali-extracted) Cellulose (residual fraction) Crude extract (unfractionated control) Key Features: - Spectral Range: 4000–400 cm⁻¹ at 4 cm⁻¹ resolution (32 scans) - Identified Functional Groups: O–H stretching (3400 cm⁻¹) Carboxylate C=O (1750 cm⁻¹, pectins) β-glycosidic linkages (890 cm⁻¹) - Correlative Data: Monosaccharide composition (GC) and antifungal activity against Penicillium sp. are linked to spectral peaks. Potential Applications: Validation of plant polysaccharide extraction protocols Structure-activity studies for natural antifungals Comparative analysis of xerophytic plant adaptations Technical Details: Files Included: FTIR-DRAOU.xlsx spectra (wavenumber vs absorbance) Instrument: Thermo Scientific FTIR spectrometer (OMNIC™ Software) Ethics: No human/animal subjects involved. This dataset supports the research article "*Structural characterization and antifungal properties of...*" [DOI/Lien manuscrit si disponible]. It contains: 1. FTIR-ATR Spectra - Raw spectra (4000-400 cm⁻¹) of mucilage (M1-M3), pectins (P1-P2), hemicelluloses, and cellulose fractions. - Key peaks: 1750 cm⁻¹ (C=O), 890 cm⁻¹ (β-glycosidic linkages). 2. Gas Chromatography (GC) - Monosaccharide composition (% molar) of all fractions. - Includes arabinose, rhamnose, xylose, galacturonic acid quantifications. 3. Colorimetric Assays - Neutral sugars (Dubois method) and uronic acids (Blumenkrantz method). - Corrected values using Montreuil-Spik equations. 4. Antifungal Bioassays - *Penicillium* sp. growth inhibition data (dose-response, IC50). Methods: - Extraction: Sequential water/EDTA/KOH protocol. - GC: Shimadzu GC-2030 (DB-5 column, TMS derivatization). - FTIR: Nicolet iS50, 4 cm⁻¹ resolution. Usage Notes: - Negative uronic acid values in cellulose set to 0. - Missing data = "ND" (Not Detected).