RNA-seq analysis of MEK or ERK1/2 inhibitor treatment in uveal melanoma cell lines.

Activating GNAQ/GNA11 are present in majority of uveal melanomas resulting in downstream activation of the MAPK pathway. To elucidate changes in the transcriptome after MEK or ERK1/2 inhibition, we performed RNA sequencing on two UM cell lines. Differential analysis revealed that MARK3 expression was not altered but STK10 expression was reduced. RNA was obtained from PDX4 and UM004 after drug treatment for 24 hours in uveal melanoma cell lines.