Xoo Effector TalAE73-Targeted OsLTPL23 Mediates Bacterial Blight Resistance in Rice

Transcription activator-like effectors (TALEs) secreted from Xanthomonas oryzae pv oryzae (Xoo) function as a pathogenicity factor to activate rice bacterial blight (BB) susceptibility, conforming to the gene-for-gene paradigm as well as resistance. Xoo pathotypes generally harbor one to three major TALEs targeting OsSWEET genes to determine pathogenicity; conversely, the immunity events mediated by minor TALEs have not been taken seriously. Here, we demonstrated that lipid transfer protein encoding gene OsLTPL23 positively regulates rice resistance to Xoo pathotype PXO61, and TalAE73PXO61, a representative member of the most widely distributed TALE family in 135 Xoo isolates, transcriptionally activates OsLTPL23 expression. Further, TalAE73PXO61 is an avirulence protein, causing effector-triggered immunity in compatible rice-Xoo interaction. In addition, reactive oxygen species accumulation, nitrate uptake, and salicylic acid homeostasis are transcriptionally and physiologically associated with OsLTPL23-dependent BB resistance.