BRR RNASeq collection - Cotton

Black root rot (BRR) disease of is an economically important disease of cotton and other crops, especially in cooler regions with short seasons. Symptoms include black discoloration of the roots, reduced number of lateral roots, and stunted or slow growth. The cultivated tetraploid species of cotton are susceptible. Resistance to BRR was identified in the G. arboreum accession BM13H and associated with reduced and restricted hyphae growth and sporulation. Transcriptome analysis indicated BM13H responded to infection at early time points 2- and three-days post inoculation, but that by day 5, few differentially expressed genes were observed between infected and uninfected roots. Inheritance of BM13H resistance to BRR was analysed in an F6 recombinant inbred population and was found to be conferred by a single dominant locus that was fine mapped to a region on chromosome 1, containing ten genes including five putative resistance-like genes.

黑色根腐病（Black root rot, BRR）是棉花及其他作物的重要经济病害，尤其在低温短季种植区发生严重。其症状表现为根系发黑变色、侧根数量减少以及植株生长迟缓或矮化。栽培四倍体棉种均对该病害敏感。在树棉（Gossypium arboreum）种质BM13H中鉴定得到对BRR的抗性，该抗性与菌丝生长受抑、扩展受限及产孢量降低密切相关。转录组分析显示，BM13H在接种后2天和3天的早期侵染阶段即产生响应，但至接种后第5天时，染病根系与未染病根系间已几乎检测不到差异表达基因。研究人员通过F6重组自交系群体对BM13H的BRR抗性遗传规律进行分析后发现，该抗性由单个显性位点调控；该位点已被精细定位至1号染色体的一段区域内，该区域包含10个基因，其中5个为潜在类抗性基因。