A cost-effective RNA sequencing protocol for large-scale gene expression studies

We present a simple RNA sequencing protocol with substantially reduced costs. Overall design: This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. H1p16 (3 replicates) and H1p43 (3 replicates) are sequenced by Illumina TruSeq. Lane1 - Lane 4 are sequenced by LM-Seq (Hou, Zhonggang, et al. 2015). Lane 1 - Lane 4 samples are multiplexed by 95, 48, 24 and 6 samples per lane, respectively.