EV based miR-6772-5p secreted by breast cancer cells in response to radiation exposure is involved in development of radioresistance

This dataset serves as a supplementary material for the research article EV-based miR-6772-5p secreted by breast cancer cells in response to radiation exposure is involved in development of radioresistance by Tynjälä et al. For further details, please refer to our publication for a detailed description of the methods used to generate this dataset.

In summary, we isolated and sequenced miRNAs from extracellular vesicles (EVs) isolated from irradiated and non-irradiated MCF7 cells. Sequencing libraries were prepared with QIAseq miRNA Library Kit (Qiagen, Germany) and sequenced with Illumina NextSeq 500 platform (Illumina). In-house bioinformatics workflow was used to process the sequencing data. Unique molecular identifier (UMI) sequences were first extracted and added to FASTQ header with UMI-tools while discarding the 3’ adapter and primer sequences. Reads shorter than 16 bp were discarded with cutadapt. Provided sequencing data is in FASTQ format and trimmed from adapter and UMI sequences. We have also provided miRNA counts and results of following differential gene expression analysis in tab-delimited format.