H2S microsensor profiling conducted alongside the destructive sampling of Chesapeake Bay sediments during incubation experiments at Horn Point Lab

These data were collected as part of a sediment incubation experiment, to investigate potential interactions between estuarine cable bacteria and their associated microbial community. Sediments were collected from the main channel of Chesapeake Bay at a mesohaline station that experiences seasonal oxygen depletion. The upper 10 centimeters (cm) of sediment was homogenized under anaerobic conditions, packed into polycarbonate core liners, and incubated in a dark climate-controlled room in aerated aquaria with artificial seawater (S=15.5; T=16 degrees Celsius). In a sub-set of core tubes, a 0.2 micron polycarbonate filter was held embedded at 0.5 cm depth, to block the downward growth of cable bacteria while allowing for porewater diffusion. The sediments (with and without embedded filters) were destructively sampled on 6 dates over 46 days, at which time nucleic acids, samples for microscopy, and samples for porewater geochemistry were collected. Microsensor profiling (oxygen (O2), pH, hydrogen sulfide (H2S)) was conducted alongside the destructive sampling. Associated nucleic acid data are available through NCBI Sequence Read Archive, under Bioproject PRJNA833464. Sediment porewaters were handled anaerobically, and were extracted by centrifugation followed by filtration (0.2 micron in-line filters). Geochemical data include porewater anions (sulfate and chloride) measured by ion chromatography, and ammonium and ferrous iron concentrations measured by standard colorimetric methods. Microscopy data include both counts of cable bacteria measured with FISH oligoprobe DSB706, and total single cell bacteria. The dataset was generated by Pinky Liau under the supervision of Dr. Sairah Malkin, Horn Point Laboratory, U. Maryland Center for Environmental Sciences.本数据集源自一项沉积物培养实验，旨在探究河口电缆细菌（cable bacteria）与其关联微生物群落间的潜在相互作用。沉积物采自切萨皮克湾（Chesapeake Bay）主河道的一个中盐度站位，该站位存在季节性缺氧现象。取表层10厘米（cm）沉积物，在厌氧条件下均质化后装填至聚碳酸酯（polycarbonate）岩心衬管中，随后置于装有人工海水（盐度S=15.5，温度T=16℃）的充气水族箱内，于避光控温的实验室环境中培养。在部分岩心管中，于0.5厘米深度处嵌入0.2微米聚碳酸酯滤膜，以阻断电缆细菌向下生长，同时允许孔隙水扩散。在46天的实验周期内，分6个时间点对装有/未装有嵌入滤膜的沉积物进行破坏性采样，采集用于核酸分析、显微镜观测及孔隙水地球化学分析的样品。破坏性采样同步开展微传感器剖面测量，检测指标包括氧气（O₂）、pH值及硫化氢（H₂S）。相关核酸数据可通过美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）序列读取档案（Sequence Read Archive）获取，对应生物项目编号为PRJNA833464。沉积物孔隙水全程厌氧处理，先通过离心提取，再经0.2微米在线滤膜过滤。地球化学数据涵盖通过离子色谱法测定的孔隙水阴离子（硫酸盐与氯化物），以及采用标准比色法测定的铵盐与亚铁离子浓度。显微镜观测数据包含采用荧光原位杂交（FISH）寡核苷酸探针DSB706计数的电缆细菌数量，以及总单细胞细菌计数。本数据集由Pinky Liau在马里兰大学环境科学中心霍恩角实验室（Horn Point Laboratory, University of Maryland Center for Environmental Sciences）的Sairah Malkin博士指导下完成。