EMDataResource

Cryo electron microscopy enables the determination of 3D structures of macromolecular complexes and cells from 2 to 100 Å resolution. EMDataResource is the unified global portal for one-stop deposition and retrieval of 3DEM density maps, atomic models and associated metadata, and is a joint effort among investigators of the Stanford/SLAC CryoEM Facility and the Research Collaboratory for Structural Bioinformatics (RCSB) at Rutgers, in collaboration with the EMDB team at the European Bioinformatics Institute. EMDataResource also serves as a resource for news, events, software tools, data standards, and validation methods for the 3DEM community. The major goal of the EMDataResource project in the current funding period is to work with the 3DEM community to (1) establish data-validation methods that can be used in the process of structure determination, (2) define the key indicators of a well-determined structure that should accompany every deposition, and (3) implement appropriate validation procedures for maps and map-derived models into a 3DEM validation pipeline.

冷冻电子显微镜技术使得从2至100埃的分辨率下确定大分子复合物和细胞的3D结构成为可能。EMDataResource是一个统一的全球门户，提供一站式存档和检索3D电子密度图、原子模型及其相关元数据的服务，它是斯坦福/SLAC冷冻电子显微镜设施与罗格斯大学研究生物信息结构合作实验室（RCSB）的联合项目，并与欧洲生物信息学研究所的EMDB团队协作。EMDataResource还作为3D电子显微镜社区新闻、活动、软件工具、数据标准和验证方法的信息资源。在当前的资金周期内，EMDataResource项目的主要目标是与3D电子显微镜社区合作，(1) 建立可用于结构确定过程的数据验证方法，(2) 确定每次存档应伴随的确定结构的关键指标，以及(3) 将适当的验证程序应用于地图及其派生模型，并将其纳入3D电子显微镜验证流程。