Fatty acid profiles of M. menidia females and their unfertilized eggs.

<p><span style=\"font-size:14px\"><span style=\"font-family:arial,helvetica,sans-serif\"><span style=\"color:rgb(0, 0, 0)\">Gas chromatography was used to quantify the absolute (mg g dry weight-1) and relative concentrations (% of total) of 27 FAs for each of 5 females (whole individual) and their unfertilized eggs (~ 1 ml). Samples were first dried and then homogenized in a solution of chloroform-methanol (2:1 v/v) and tricosanoic acid (23:0) as an internal standard for quantification of mg g−1&nbsp;dry mass of fatty acids. Lipids were cold-extracted from approximately 50 mg of dry mass. A Shimadzu GC-2014 gas chromatograph set with a Phenomenex ZB-WAX plus capillary column (30&nbsp;m long; 0.53 mm ID; 1.0 um thick) was used to quantify FAs, and individual FAs were identified by comparison to commercial standards (Supelco, Inc).&nbsp;</span></span></span></p>

<p><strong>These data are&nbsp;associated with the corresponding paper:</strong></p>

<p><a href=\"https://doi.org/10.1016/j.jembe.2017.11.002\" target=\"_blank\">Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. <em>Journal of Experimental Marine Biology and Ecology</em> (in press).</a></p>

<p><strong><span style=\"font-size:14px\">Other datasets related to this paper:</span></strong></p>

<p><a href=\"https://www.bco-dmo.org/dataset/719420\" target=\"_blank\">Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments.</a></p>

<p><span style="font-size:14px"><span style="font-family:arial,helvetica,sans-serif"><span style="color:rgb(0, 0, 0)">本研究采用气相色谱法（Gas Chromatography）定量分析了5只雌性成体（完整个体）及其未受精卵（约1mL）中27种脂肪酸（Fatty Acids，FAs）的绝对浓度（单位为mg·g⁻¹干重）与相对浓度（占总脂肪酸的百分比）。实验流程为：样品先经干燥处理，随后在氯仿-甲醇（体积比2:1）与二十三烷酸（23:0，作为内标（internal standard））的混合溶液中均质化，用于测定脂肪酸的干重浓度（mg·g⁻¹）。脂质从约50mg干重样品中通过冷提取法获取。本实验采用岛津（Shimadzu）GC-2014气相色谱仪，搭配Phenomenex ZB-WAX plus毛细管柱（柱长30m、内径0.53mm、膜厚1.0μm）完成脂肪酸定量，并通过与商业标准品（Supelco公司）比对鉴定单个脂肪酸组分。</span></span></span></p><p><strong>本数据集关联于以下相关论文：</strong></p><p><a href="https://doi.org/10.1016/j.jembe.2017.11.002" target="_blank">Snyder, J.T.*、Murray, C.S.*与Baumann, H.（2017）：《沿岸海水鱼类母本效应对子代CO₂敏感性的潜在影响》，刊载于《实验海洋生物学与生态学杂志》（已录用待刊）。</a></p><p><strong><span style="font-size:14px">本文相关其他数据集：</span></strong></p><p><a href="https://www.bco-dmo.org/dataset/719420" target="_blank">不同雌性亲本的M. menidia子代在不同CO₂环境下的存活、体长与生长响应。</a></p>