Characterization of bronchiolitis and vaccine-induced enhanced respiratory disease in Syrian hamsters caused by respiratory syncytial virus infection

This dataset comprises experimental records from a controlled laboratory study of respiratory syncytial virus (RSV) infection in Syrian hamsters (Mesocricetus auratus) , and induced enhanced respiratory disease (ERD)following immunization with heat-inactivated RSV (HI-RSV). Data on RSV infection were collected from three age groups (neonatal: 3 days old; adult: 6–8 weeks old; old: 70–80 weeks old), with 5–6 animals in each group. Data on immunization were collected from old hamsters immunized with HI-RSV (HI-RSV), heated non-infected Hep-2 cell lysate (mock), or aluminium adjuvant with PBS (PBS), before infection with RSV (5–6 animals in each group). Additionally, 70–80-week-old BALB/c mice and 6–8-week-old cotton rats were infected with RSV, and data were obtained from these infections (3-6 animals in each group). The package includes: Clinical indicators: Daily percentage change in body weight and standardised clinical symptom scores (scale 0–3). Viral replication data: Lung viral load quantified by TaqMan probe-based qPCR (standard curve method; copies/μg RNA), Viral titres from lung homogenates (TCID₅₀/g; measured in HEp-2 cells). Haematology: Complete blood counts (CBC) with differentials (WBC, lymphocytes, neutrophils, monocytes, eosinophils and basophils). Histopathology: Semi-quantitative scores (grade 0–4) of lung inflammation, necrosis and peribronchiolar infiltration from haematoxylin and eosin (H&E)-stained sections. Transcriptome data: gene expression data, differentially expressed genes (DEGs), functional enrichment analyses of DEGs. Primers list: RT-qPCR primer sequences for validating the expression levels of differentially expressed genes (DEGs). All procedures were approved by the Institutional Animal Care and Use Committee of the Institute of Laboratory Animal Science, Peking Union Medical College (ILAS, PUMC) (LJN-23005). This resource supports research on RSV pathogenesis, age-dependent immune responses, and the evaluation of vaccine efficacy.本数据集涵盖一项受控实验室研究的实验记录，该研究包含两部分内容：叙利亚仓鼠（Mesocricetus auratus）的呼吸道合胞病毒（respiratory syncytial virus, RSV）感染模型，以及经热灭活RSV（heat-inactivated RSV, HI-RSV）免疫后诱发的加重型呼吸道疾病（enhanced respiratory disease, ERD）模型。 研究中，RSV感染相关数据采集自三个年龄分组：新生组（3日龄）、成年组（6~8周龄）及老年组（70~80周龄），每组包含5~6只实验动物。免疫相关数据则采集自经HI-RSV免疫的老年仓鼠（HI-RSV组）、加热灭活的未感染HEp-2细胞裂解物免疫的老年仓鼠（模拟免疫组，mock组）以及仅搭载磷酸盐缓冲液（PBS）的铝佐剂免疫的老年仓鼠（PBS组）；所有免疫组均在免疫完成后接受RSV感染，每组包含5~6只实验动物。 此外，本数据集还纳入了70~80周龄的BALB/c小鼠及6~8周龄的棉鼠的RSV感染相关数据，每组包含3~6只实验动物。 本数据集包含以下内容： 1. 临床指标：每日体重变化百分比与标准化临床症状评分（评分区间0~3）。 2. 病毒复制相关数据： ① 肺脏病毒载量：采用基于TaqMan探针的实时荧光定量PCR（quantitative PCR, qPCR）进行定量，检测方法为标准曲线法，单位为拷贝/微克RNA； ② 肺组织匀浆病毒滴度：以TCID₅₀/克为单位，采用HEp-2细胞进行测定； 3. 血液学指标：全血细胞计数（complete blood counts, CBC）及白细胞分类计数，涵盖白细胞总数（white blood cell count, WBC）、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞与嗜碱性粒细胞； 4. 组织病理学数据：苏木精-伊红（haematoxylin and eosin, H&E）染色切片的肺脏炎症、坏死及细支气管周围浸润半定量评分，评分区间为0~4； 5. 转录组数据：基因表达谱数据、差异表达基因（differentially expressed genes, DEGs）及差异表达基因的功能富集分析结果； 6. 引物序列列表：用于验证差异表达基因表达水平的逆转录实时荧光定量PCR（reverse transcription quantitative PCR, RT-qPCR）引物序列； 所有实验操作均经北京协和医学院实验动物研究所实验动物伦理委员会（Institutional Animal Care and Use Committee of the Institute of Laboratory Animal Science, Peking Union Medical College, ILAS, PUMC）批准，审批编号为LJN-23005。 本数据集可用于呼吸道合胞病毒致病机制、年龄依赖性免疫应答及疫苗效力评估相关研究。