Proteins with significant expression differences in spleen leukocytes of 2 week and 4 week old NOD mice compared to two control strains and their expression differences at RNA level.

Statistical analysis of protein expression data by 1-way-ANOVA (p<0.05) followed by hierarchical clustering identified 11 and 8 spots that were differentially expressed in spleen leukocytes of 2 week and 4 week old NOD mice, respectively, compared to two control strains (NON and C57BL/6); H, indicates spots that were of higher expression in NOD, all other spots were of lower expression in NOD mice. We identified proteins in 9 and 7 of the spots at 2 and 4 weeks, respectively. We also analyzed expression differences of the identified proteins at the RNA level using the microarray (mRNA) dataset; NS, indicates no significance in gene expression between NOD and control strains. Fold change (FC) was calculated by ratio of means of expression in NOD mice versus controls; FC of spots/genes of lower expression in NOD are indicated by negative values.