The roles of Heme oxygenase-1 roles in the analgesic effects of pregabalin and gabapentin in neuropathic pain mice model

This dataset is the results of investigation of the effects of Heme oxygenase-1 inhibitor, tin protoporphyrin IX (SnPP) or zinc (II) protoporphyrin IX (ZnPP), on the analgesic effects of pregabalin and gabapentin. We also examined the effects of co-administration of SnPP with pregabalin or gabapentin on the expression of glial markers or other genes. Neuropathic pain was developped using spared nerve injury of the sciatic nerve in C57BL6 male mice. The mechanical threshold was determined by using the von Frey filaments. The expression of spinal glial markers or other genes was studied using reverse transcription polymerase chain reaction. Heme oxygenase-1 plays a role in the analgesic efficacy of calcium channel α2δ ligands through the attenuation of glial activation and endogenous opioid release.

本数据集为血红素氧合酶-1（Heme oxygenase-1）抑制剂锡原卟啉IX（SnPP）或锌(II)原卟啉IX（ZnPP）对普瑞巴林与加巴喷丁镇痛作用影响的研究结果。本研究同时考察了锡原卟啉IX与普瑞巴林或加巴喷丁联合给药对胶质细胞标志物及其他基因表达的影响。本研究通过对C57BL6雄性小鼠实施坐骨神经保留性神经损伤（spared nerve injury）以构建神经病理性疼痛模型。机械痛阈值采用冯·弗雷纤维丝（von Frey filaments）法测定。脊髓胶质细胞标志物及其他基因的表达水平通过逆转录聚合酶链式反应（reverse transcription polymerase chain reaction，RT-PCR）进行检测。血红素氧合酶-1可通过抑制胶质细胞活化与内源性阿片肽释放，参与钙通道α2δ配体的镇痛效能调控。