Hydrolysis and transglycosylation activities of Endo-A mutants.
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aThe hydrolysis activity was determined using Ribonuclease B as the substrate, the transglycosylation activity was measured using a chitinase-coupled assay, in which Man9GlcNAc2Asn-Fmoc was used as the donor substrate and 4MU-GlcNAc as the acceptor. bND, not detected.
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2009-03-02
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