A comparison of strategies for mixing WGBS libraries

The nucleotide composition of sequencing library for whole-genome bisulfite sequencing (WGBS) is extremely biased. This causes inaccurate base calling on Illumina's sequencers. To overcome this, we conceived a strategy that mixes two libraries of the reciprocal polarities for sequencing direction. We compared this strategy with a conventional one that mixes WGBS library with a library of normal nucleotide composition called PhiX.