HIFI-Barcode-se400

Here we introduce a single-end 400bp sequencing strategy in BGISEQ-500 platform, which allow us to generates full-length reference barcodes via simply connection of 5' and 3' reads. Briefly, individual genomic DNA was extracted separately and amplified on a 96-well plate using 96 sets of uniquely tagged primers. Amplicons were then pooled, without fragment, and sequenced on BGISEQ-500 platform at 400 SE. Mixed HTS reads were assembled using a customized bioinformatics pipeline to obtain the barcode sequence for each individual. Compared with the available HTS platforms, our method had a more concise library construction strategy and easier analysis practice and most cost-effective.