Fast kinetics data of binding of azide to myoglobin, training data -- MetBio practicals

These data files were acquired during the course of the 3rd FrenchBIC MetBio summer school taking place in and around Marseille. The data were acquired during the “stopped flow” practicals. The data correspond to the reaction of Myoglobin with various concentrations of azide, triggered using a stopped-flow apparatus and followed by UV/Visible spectroscopy. The concentrations of azide can be deduced from the names of the files and are expressed in millimolar. The concentrations of myoglobin are variable but always much lower than that of azide. The purpose of this dataset is to be used as training data for analysing multiwavelength kinetic data. It will be the subject of a data analysis tutorial using the free software QSoas, to be published there. With the exception of the <code>WTV-Azide-0.5mm_3.txt</code>, all the files are in a “matrix” format, in which the first column gives the time and each column after the first corresponds to the absorbances over time of a single wavelength. The <code>WTV-Azide-0.5mm_3.txt</code> uses a different format, in which each line corresponds to a <em>wavelength</em> <em>time</em> <em>absorbance</em> triplet. Useful background reading: Coletta <em>et al</em>, <strong>1996</strong>, DOI: 10.1111/j.1432-1033.1996.00049.x De Sanctis <em>et al</em>, <strong>2007</strong>, DOI: 10.1529/biophysj.106.098764本数据集文件采集于在马赛市内及周边举办的第三届法国生物信息与计算生物学中心（FrenchBIC）代谢生物学（MetBio）暑期学校期间，相关数据取自课程中的“停流法”实操环节。本数据集对应肌红蛋白（Myoglobin）与不同浓度叠氮化物的反应：实验通过停流装置触发反应，并利用紫外-可见（UV/Visible）分光光度法追踪反应进程。叠氮化物的浓度可通过文件名推导得出，单位为毫摩尔每升（mmol/L）；肌红蛋白的浓度存在波动，但始终远低于叠氮化物的浓度。本数据集旨在作为多波长动力学数据分析的训练数据集使用，后续将依托免费软件QSoas开设数据分析教程并公开发布。除<code>WTV-Azide-0.5mm_3.txt</code>外，所有文件均采用“矩阵”格式：第一列为时间数据，后续每一列分别对应单一波长下随时间变化的吸光度值；而<code>WTV-Azide-0.5mm_3.txt</code>采用了不同格式，每一行均为一组<em>波长</em>、<em>时间</em>与<em>吸光度</em>三元数据。相关背景参考文献：科莱塔（Coletta）等人，1996年，DOI：10.1111/j.1432-1033.1996.00049.x；德桑克蒂斯（De Sanctis）等人，2007年，DOI：10.1529/biophysj.106.098764