Microbiome-dependent tryptophan metabolism suppresses inflammatory immunity in the pancreatic tumor microenvironment by activation of the aryl hydrocarbon receptor [RNA-seq]

To explore the role of macrophages and AhR in PDAC, we used an orthotopic model employing mouse PDAC organoid lines derived from a primary tumor isolated from the KPC model of PDAC and performed RNA-sequencing on FACS-sorted TAM from 14-day tumors and compared them to the residential macrophages from normal pancreas. Gene expression profiling of sorted macrophages (i.e. resident Macrophages, n=4 mice) or PDAC tumors from control (n=3) or Lyz2-AhR cKO (n=3) mice 14 days after tumor implantation.