CyTOF T1D B cell normalized data set

Insulin and Tetanus binding B cells were collected via MACS sorting for analysis using CyTOF (cytometry by time of flight). Data was then analyzed using unsupervised clustering and manual gating to find unique phenotypes and populations associated with recent-onset individuals with type 1 diabetes (T1D) compared to age-matched healthy controls (HC)

通过磁性活化细胞分选（Magnetic Activated Cell Sorting, MACS）收集结合胰岛素与破伤风抗原的B细胞，以用于飞行时间质谱流式细胞术（CyTOF, cytometry by time of flight）分析。随后采用无监督聚类与手动门控技术对所得数据进行解析，旨在相较于年龄匹配的健康对照（healthy controls, HC），鉴定出与新发1型糖尿病（type 1 diabetes, T1D）患者相关的特异性细胞表型与细胞群。