MALDI-FT-ICR-MS data for ZooMS analysis of Phasianidae remains from the Namjeon shell midden, South Korea
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This dataset contains raw MALDI-FT-ICR-MS data for the ZooMS analysis of Phasianidae remains from the Namjeon shell midden, South Korea. Methodological Notes: Collagen was extracted using a standard acid-insoluble protocol. Samples were demineralized in HCl and gelatinized at 65 ℃ in AmBic. Following gelatinization, samples were digested with trypsin. Peptides were purified using C18 ZipTips for MALDI-FT-ICR-MS analysis. Further proteomic data will be accessible via ProteomeXchange. File Description: 1. Sample List.xlsx Metadata for all specimens analyzed in this study, including sample IDs, period, element, and taxonomic information for both modern references and archaeological samples from the Namjeon shell midden. 2. MALDI_Namjeon_samples.zip This file includes unprocessed .mzML spectra files for specimens from the Namjeon site, acquired via MALDI-FT-ICR-MS. 3. Namjeon_Sample_Spectra.zip This file contains visual representations (spectral images) of the Phasianidae specimens from the Namjeon site. Corresponding LC-MS/MS data can be found at MassIVE (DOI: 10.25345/C5RR1Q17T)
本数据集包含韩国南田贝冢出土的雉科(Phasianidae)遗存进行动物考古质谱(ZooMS)分析所需的原始基质辅助激光解吸电离傅里叶变换离子回旋共振质谱(MALDI-FT-ICR-MS)数据。 方法学说明:采用标准酸不溶法提取胶原蛋白。样品经盐酸脱矿处理后,于65℃下在碳酸氢铵(AmBic)缓冲液中进行凝胶化。凝胶化完成后,使用胰蛋白酶对样品进行酶解。酶解得到的肽段经C18 ZipTips纯化后,用于MALDI-FT-ICR-MS分析。后续蛋白质组学数据将通过ProteomeXchange平台公开获取。 文件说明: 1. Sample List.xlsx:本研究所有分析标本的元数据,涵盖现代对照样本与南田贝冢出土考古样本的样本编号、文化时期、出土部位以及分类学信息。 2. MALDI_Namjeon_samples.zip:该文件包含南田遗址出土标本未经处理的.mzML格式质谱谱图文件,均通过MALDI-FT-ICR-MS采集获得。 3. Namjeon_Sample_Spectra.zip:该文件包含南田遗址出土雉科标本的质谱谱图可视化图像。 相关液相色谱-串联质谱(LC-MS/MS)数据可在MassIVE数据库获取(DOI:10.25345/C5RR1Q17T)



