Structure and degradation of circular RNAs regulate PKR activation in innate immunity

RNase L–mediated rapid degradation of circRNAs is required for PKR activation in innate immune responses and linked to the autoimmune disease systemic lupus erythematosus. Overall design: Poly(A)+ RNA-seq for gene expression, poly(A)–/Ribo– for circRNA, circSHAPE-MaP and linearSHAPE-MaP for circRNA and linear RNA secondary structure, poly(A)+ RNA-seq for gene expression, poly(A)–/Ribo– for circRNA.