Mapping the mouse developmental and aging atlas by single cell mRNA-seq

Single-cell mRNA sequencing (mRNA-seq) technologies are reshaping the current cell-type classification system. In previous studies, we built a comprehensive mouse cell atlas to catalog all cell types by collecting scRNA-seq data in the fetal and adult stages. Howerver, systematically study for organism-level dynamic changes of cellular states across mouse life span are still lacking. Here, We made an updated version of mouse cell atlas (MCA) by adding scRNA-seq data covering 14 major mouse organs during different mouse development period. We revealed aging related regulatory networks and pathways that have not been well characterized previously. We found that the expressions of immune-related genes, such as antigen-presenting genes and immunoglobulin genes, appeared in non-immune cell types in aging process. We also focused on the expression of lung epithelial immunoglobulin genes and revealed their related transcriptional regulation mechanisms. The updated MCA resource provides a valuable resource for studying mammalian development, maturation and aging. Overall design: Microwell datasets for ten mouse tissues (brain, heart, intestine, kidney, liver, lung, pancreas, stomach, testis and uterus) covering 8 stages including E14.5, neonatal, 10d, 3w, 6-8w, 12m, 18m and 24m, four mouse tissues (bladder, spleen and prostate) covering aging stages including 6-8w, 12m, 18m, 24m, one tissue (thymus) covering aging stages including 6-8w, 12m, 18m and some mouse tissues used in GSE134355 and GSE108097.