Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [DA_PDF]. Drosophila melanogaster

We identified ARGs in a genome-wide manner, in fly brains as well as in sorted neurons; they included dopaminergic neurons (DA) and a subset of circadian-related neurons (PDF+ neurons). Overall design: TH-GAL4 (DA neurons)/PDF-gal4 (PDF+ cells);UAS-TrpA1 or TH-GAL4 flies stimulated with temperature shift and mRNA isolated and sequenced 0 or 60 min after.