Burkholderia spp. DNA specificity panel used in this study.

aAccording to genotyping results generated in the current study, 16 S sequencing or MLST.bPreviously identified as ‘Burkholderia spp.’ and renamed Burkholderia thailandensis/B. thailandensis-like/B. oklahomensis based on their genotyping outcomes in this study. However, we could not accurately differentiate these three species as they all amplify the non-B. pseudomallei probe in the 122018 and 266152 assays. Neither TTS1 nor BurkDiff assays amplify B. thailandensis, B. thailandensis-like or B. oklahomensis species, and thus could not be used for species assignment.cRuled out as being B. pseudomallei, B. thailandensis, B. thailandensis-like, B. oklahomensis, B. vietnamiensis and B. ubonensis but strongly suspected to be Burkholderia spp. due to their amplification using B. vietnamiensis and B. ubonensis-specific assays (Price et al., unpublished data).