Towards 3D structure determination and dynamics of biological and non-biological samples using TR-SSX methods

This BAG proposal aims to conduct room temperature time-resolved serial crystallography experiments at the new ID29 beamline at ESRF. We intend to collect
full data sets on microcrystals of two medically relevant proteins, the penicillin binding protein 2a (PBP2a) of methicillin resistant staphyloccocus aureus (MRSA)
and the human the NAP(P)H:quinone oxidoreductase 1 (NQO1) protein, with their respective ligands. As well, we aim at conducting pump-probe time-resolved
crystallography experiments on microcrystals of metal organic frameworks (MOFs). The good quality of all crystalline samples has been proved either at ESRF
beamlines ID30-A1 and ID30-A3, or at other synchrotron beamlines. At ID29, they offer a variety of sample delivery devices including high viscosity injectors, tape
drives, fixed target supports, and microfluidic devices, that will be employed in our experiments depending on sample needs.