TABLE 8 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures

TABLE 8 Binomial mixed model to assess if method (WGS or amplicon sequencing) has a significant effect on the proportion of false-positive sequencing reads identified in a pollen mixture. The direction of the trend was always in favor of amplicon sequencing DNA barcodeTaxonomic levelp -valueNrbcLSpecies.506717654Genus.691751354ITS2Species.910274054Genus.000045454rbcL and ITS2 combinedSpecies.9694023106Genus.0993345106表8 用于评估方法（全基因组测序（Whole Genome Sequencing，WGS）或扩增子测序）是否对花粉混合物中鉴定得到的假阳性测序reads比例存在显著影响的二项混合模型。该趋势始终有利于扩增子测序。 该模型的统计结果如下表所示，各列依次为DNA条形码（DNA barcode）、分类层级（Taxonomic level）、p值（p-value）及样本量： | DNA条形码（DNA barcode） | 分类层级（Taxonomic level） | p值（p-value） | 样本量 | | ----------------------- | -------------------------- | ------------- | ------ | | rbcL | 物种级 | 0.5067 | 17654 | | rbcL | 属级 | 0.6917 | 17654 | | ITS2 | 物种级 | 0.9102 | 274054 | | ITS2 | 属级 | 0.000045454 | 45454 | | rbcL与ITS2联合 | 物种级 | 0.9694 | 23106 | | rbcL与ITS2联合 | 属级 | 0.0993 | 345106 |