Peripheral clonal expansion of T lymphocytes associates with tumour infiltration and response to cancer immunotherapy

We performed single-cell RNA sequencing (scRNA-seq) and deep analysis of T cell receptor (TCR) clonotypes (scTCR-seq) in cancer patients across several indications, assessing the profiles of TCRs in the various populations of T cells in tumours, normal adjacent tissue (NAT), and peripheral blood. In a subset of patients, we find clear evidence of clonotypic expansion of T effector-like cells not only within the tumour but also in NAT. Single-cell RNA-seq and single-cell TCR-seq of pretreatment samples from 14 cancer patients, taken from tumor, normal adjacent tissue, and peripheral blood ------------------------------------------------------ The authors have submitted the raw data to EGA: EGAS00001003993 (scRNA-seq) and and EGAS00001003994 (scTCR-seq)