Long noncoding RNA expression profiles analysis in GBM patient-derived cells stimulated with interferon gamma

Long noncoding RNAs (lncRNAs) are key regulators of gene expression both in physiological and pathological conditions. Previous studies showed changes in lncRNA expression during tumorigenesis. To analyze the role of lncRNAs in tumor interferon signaling and cancer-mediated immunosuppression, we used RNA-seq technique to identify lncRNAs that are differentially expressed in GBM patient-derived cells stimulated with interferon gamma compared to unstimulated cells. 15,768 lncRNAs were surveyed in the analysis. RNA-seq data show upregulation of 113 lncRNAs upon interferon gamma treatment (p value < 0.01, fold change > 2). Our findings indicate that the expression of several lncRNAs is stimulated by interferon gamma suggesting a critical role of lncRNAs in tumor interferon signaling. Patient-derived GBM cells were stimulated with interferon gamma for 24 hours. Non stimulated cells were used as control. LncRNA expression profiles were generated by RNA-seq, in triplicate, using Illumina HiSeq 4000.