Here we introduce a single-end 400bp sequencing strategy in BGISEQ-500 platform, which allow us to generates full-length reference barcodes via simply connection of 5’ and 3’ reads. Briefly, individua
aLinker sequences were designed according to Walters et al. [28]. bGolay barcodes of 12-nucleotides in length were used in combination with this primer. cHamming barcodes of 8-nucleotides in length we