Metagenomics Sequencing Data related to article "Translocation and dissemination of commensal bacteria in post-stroke infection"

Two pieces of sequencing data under the accession codes 4675190.3 and 4707925.3 are held at the MG-RAST Metagenomics Analysis Server

Synopsis of related article is below:

Bacterial pneumonia is highly prevalent in stroke patients, and it is widely believed that overt of micro-aspiration is the primary cause of pneumonia among stroke patients. In this study, we demonstrated that post-stroke infection was only observed in mice raised in specific pathogen free (SPF) facilities, and not germ free (GF). We used high throughput 16S rRNA gene amplicon sequencing and relevant bioinformatics tools to determine that the likely sources of bacteria that form the microbial community in the post-stroke lung are the commensal species from the host small intestine. The peripheral dissemination of bacteria inoculated orally was only seen in post-stroke mice. Our results therefore provide evidence to indicate that bacterial infections following stroke are likely to originate endogenously from the host commensal microbiota. Submitted is Qiime processed split library fasta file with all samples strting with S being sham and with T stroke mice.

编号为4675190.3与4707925.3的两组测序数据，存储于MG-RAST宏基因组分析服务器中。
相关研究文章的内容提要如下：
细菌性肺炎在脑卒中患者中高发，学界普遍认为微误吸是脑卒中患者罹患肺炎的主要诱因。本研究证实，脑卒中后感染仅见于饲养于无特定病原体（specific pathogen free, SPF）环境中的小鼠，而无菌（germ free, GF）小鼠未出现此类感染。我们采用高通量16S rRNA基因扩增子测序技术及相关生物信息学工具，明确了脑卒中后肺部微生物群落的细菌来源为宿主小肠内的共生菌群。经口接种的细菌仅在脑卒中后小鼠体内发生外周播散。因此本研究结果表明，脑卒中后的细菌性感染大概率源自宿主自身的共生微生物群。本次提交的是经Qiime处理的拆分库fasta文件，所有命名以S开头的样本为假手术小鼠样本，以T开头的样本为脑卒中模型小鼠样本。