Testing the effect of individual scent compounds on pollinator attraction in nature using quasi-isogenic Capsella lines
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Data are available in four Appendices that are presented in Word files. APPENDIX S1. Crossing scheme for construction of scent-manipulated genotypes (<em>CNL1</em>/<em>TPS2</em>, <em>CNL1</em>/<em>tps2</em>, <em>cnl1</em>/<em>TPS2</em> and <em>cnl1</em>/<em>tps2</em>). APPENDIX S2. Primers for scent-manipulated individual genotyping and haplotype amplification. APPENDIX S3. Number of visits by different pollinator group to flowers of the four scent-manipulated genotypes and SC <em>Capsella grandiflora</em> in forty 15-min observation periods. APPENDIX S4. Haplotype-frequency dot plots for each amplicon. Dots with the same color between different individuals indicate the same haplotype sharing. CgCg: <em>CNL1</em>/<em>TPS2</em>, CgCr:<em> cnl1</em>/<em>TPS2</em>, CrCg: <em>CNL1</em>/<em>tps2</em>, CrCr: <em>cnl1</em>/<em>tps2</em> and SC: SC <em>C. grandiflora</em>. Numbers after genotype names indicate their individual and sampling plot number, for example, CgCg2_1 refers the individual No. 1 of <em>CNL1</em>/<em>TPS2 </em>genotype in sampling plot No. 2. Individuals with number 48/418 refer to the ones whose leaf materials were harvested to provide parental haplotype information.
本数据集的相关数据以Word文档形式收录于4个附录中,具体内容如下: 附录S1:4种气味修饰基因型(<em>CNL1</em>/<em>TPS2</em>、<em>CNL1</em>/<em>tps2</em>、<em>cnl1</em>/<em>TPS2</em>及<em>cnl1</em>/<em>tps2</em>)的构建杂交方案。 附录S2:用于气味修饰基因型个体基因分型与单倍型扩增的引物序列。 附录S3:在40个15分钟观测周期内,不同传粉者类群对4种气味修饰基因型及自交亲和<em>Capsella grandiflora</em>花朵的访花次数统计。 附录S4:各扩增片段的单倍型频率散点图。不同个体间颜色一致的点代表共享的同一种单倍型。 基因型代号说明: CgCg:<em>CNL1</em>/<em>TPS2</em>;CgCr:<em>cnl1</em>/<em>TPS2</em>;CrCg:<em>CNL1</em>/<em>tps2</em>;CrCr:<em>cnl1</em>/<em>tps2</em>;SC:自交亲和(Self-compatible,SC)<em>Capsella grandiflora</em>。 基因型名称后的数字为个体编号与样地编号,例如CgCg2_1代表样地2中<em>CNL1</em>/<em>TPS2</em>基因型的1号个体。编号为48/418的个体为采集叶片材料以获取亲本单倍型信息的实验材料。



