Transcriptome profile in IEC4.1 cells following Cryptosporidium parvum infection

Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children in developing countries. Infection by this parasite causes significant alterations in the gene expression profiles in infected host cells. This study aims to measure the genomic wide alterations in gene expression profiles in host intestinal epithelial cells following C. parvum infection. Mouse intestinal epithelial (IEC4.1) cells were grown to 80% confluence and exposed to C. parvum infection for 24h. Total RNA was collected for the genome-wide analysis. The Agilent SurePrint G3 mouse Gene Expression Microarray (G4852A) was used for the genome-wide analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4852A/G4852A). The IEC4.1 cells were grown to 80% confluence for two groups: control (Group A, A1-A3) and C. parvum infection (Group B, B1-B3; cells exposed to C. parvum infection for 24h). Total RNAs were prepared with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instruction.