Role of RBMX in the post-transcriptional control of homeotic genes

Wild-type and RBMX-knockdown HEK293 cells where profiled at the transcriptome and translatome levels for the expression of 50 homeotic genes. Targeted RNA-seq libraries were built and sequenced on an Illumina MiSeq machine to allow for accurate gene expression quantification of this specific set of genes. To assess the binding of RBMX to the same set of mRNAs, RNA immunoprecipitation was also performed on HIS-HA tagged RBMX expressing HEK293 cells. Overall design: 5 biological samples per condition are used for the RIP samples, 5 per condition for the knock-down samples. A total of 32 samples were sequenced.