Tumor-intrinsic P2RY6 signaling promotes prostaglandin E2 production to suppress anti-tumor immunity [ATAC]

Our RNAseq results showed that P2RY6 induced the expression of Ptgs1 and Ptgs2 in MC38 cells. To confirm whether P2RY6 increases the opening of cis-regulatory elements to promote Ptgs1/2 transcription, we performed Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) to determain the chromatin accessibility across the genome in MC38 cells with or without P2RY6 expression. Comparative profiling analysis of ATAC-seq data from control cells and P2ry6-expressing MC38 cells treated with UDP 12 hours (n=2).