Transcriptomic profiling of liver tissue from control, DHT-induced PCOS-NAFLD model, and baicalin-treated mice using RNA-seq

This study aimed to characterize the liver transcriptomic changes in a mouse model of polycystic ovary syndrome (PCOS)–related non-alcoholic fatty liver disease (NAFLD) and to assess the therapeutic effects of baicalin (BA). Female C57BL/6J mice were implanted subcutaneously with dihydrotestosterone (DHT) pellets to induce PCOS-NAFLD. Liver tissues from control, model, and BA-treated mice were collected for RNA sequencing (RNA-seq). Differential gene expression and functional enrichment analyses were performed to identify biological processes and pathways associated with disease progression and BA intervention. Liver RNA-seq was performed on female C57BL/6J mice divided into three groups: control, PCOS-NAFLD model, and BA-treated at a dose of 200 mg/kg/day (n = 4 per group).