Supplementary Data for 'The electron-proton bottleneck of photosynthetic oxygen evolution'

Supplementary data for the publication: 'The electron-proton bottleneck of photosynthetic oxygen evolution', Greife et al. Heat_Artefact_Spectrum.xlsx<br> Spectrum assigned to rapid heating by laser-flash excitation of high-activity Photosystem II membrane particles. This spectrum was used for heat artefact correction of the step-scan data set. The first column is the wavenumber in cm^-1, the second column the absorption in arbitrary OD units. Log_Averaged_Interferograms.xlsx<br> Averaged time-resolved FTIR interferograms recorded before and after the application of a saturating excitation flash to high activity Photosystem II membrane particles from spinach. In total 10 sequential flashes were applied. The first column is the time in milliseconds. Remaining columns are the mirror positions of the phase-corrected interferograms with the centerburst shifted to the first position. Amplitude is in arbitrary units. Individual columns correspond to individual time courses at the respective mirror positions. Rows corresponds to interferograms at the respective time. The data for each of the 10 flashes is provided in a separate spreadsheet, which is accordingly labeled. The mirror positions were chosen such that after Fourier transformation the wavenumber range of the resulting spectra corresponds to 929.33 cm^-1 to 1858.67 cm^-1, with data points equally spaced on a wavenumber axis.本数据集为学术论文《光合氧气释放的电子-质子瓶颈》（*The electron-proton bottleneck of photosynthetic oxygen evolution*）的补充数据，作者为Greife等人。 ### Heat_Artefact_Spectrum.xlsx 该光谱对应高活性光系统II（Photosystem II）膜颗粒经激光闪光激发产生的快速加热热伪影（heat artefact）。此光谱用于步进扫描数据集的热伪影校正。第一列为波数，单位为cm⁻¹；第二列为吸光度，采用任意光密度（Optical Density, OD）单位。 ### Log_Averaged_Interferograms.xlsx 该文件包含对菠菜来源的高活性光系统II膜颗粒施加饱和激发闪光前后记录的平均时间分辨傅里叶变换红外（Fourier Transform Infrared, FTIR）干涉图。本次实验总计施加10次连续闪光。第一列为时间，单位为毫秒（ms）；其余各列为相位校正后干涉图的镜位，其中中心脉冲（centerburst）已偏移至首个位置，振幅采用任意单位。各列分别对应不同镜位下的独立时间进程，各行则对应不同时刻的干涉图。10次闪光的单次数据分别存储于单独的电子表格中，并已进行对应标注。镜位参数经优化选取，确保傅里叶变换后所得光谱的波数范围为929.33 cm⁻¹至1858.67 cm⁻¹，且数据点在波数轴上均匀分布。