Human HFF cells large rearrangements detection after CRISPR-Cas9 nuclease (Fig. 3d). Human HFF cells large rearrangements detection after CRISPR-Cas9 nuclease (Fig. 3d)

SORSC1 gene editing by CRISPR-Cas9 nuclease in heterozygous HFF for UROS inactivation. Fluorescent positive cell sorting for UROS and large rearrangements confirmation in chromosome 10q. Overall design: Three-condition experiment, HFF vs Agilent control DNA, Two Fluorescent-positive clones after SORSC1 gene editing and bulk before editing