Field sampling and DNA-barcoding of fig pollinator wasps across host species and host developmental phase and on non-Ficus controls

To better understand factors that might contribute to this observed range of specificity, we used sticky traps to capture fig-pollinating wasp individuals at 13 Ficus species, sampling at different stages of the reproductive cycle of the host figs (e.g. trees with receptive inflorescences, or vegetative trees, bearing only leaves). We also sampled at other tree species, using them as non-Ficus controls. DNA barcoding allowed us to identify the wasps to species, and therefore assign their presence and abundance to host fig species and the developmental stage of that individual tree. Here we upload the data and the R scripts used to analyze these data.

Methods
The datasets were collected in the landscape surrounding the Barro Colorado Island field station of the Smithsonian Tropical Research Institute in Panama and DNA-bardcoding preparations were done in the NAOS laboratories of the Smithsonian Tropical Research Institute in Panama. Purification and sequencing were done in Korea (Macrogen). Data was collected from 2016–2019.