CIL:1454

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid rprs_7544196.tifhelium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 75,000x magnification. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:2874.

将兔腰大肌骨骼肌纤维维持于恒定长度，并通过浸入甘油诱导其进入肌肉僵直状态。将纤维与液氦冷却的铜块接触，通过Heuser型冷冻压样器实现快速冷冻，随后在巴尔泽斯（Balzers）400型冷冻断裂蚀刻机中进行冷冻蚀刻处理。之后以铂-碳（Pt-C）合金在24°条件下对样本进行旋转复型，再使用工作电压为100千伏的日本电子（JEOL）100CX2型透射电子显微镜进行成像观测。成像采用柯达（Kodak）4489胶片，放大倍率为75000倍。随后使用尼康（Nikon）Coolscan 9000ED扫描仪以20微米的像素间距对胶片进行扫描。该立体图像对的编号为CIL:2874。