Characterization of human non-hematopoietic bone marrow cells in non-fibrotic and fibrotic conditions

We isolated non-hematopoietic cells from fibrotic and non-fibrotic human bone marrow and perfomed scRNAseq on them. We identified 3 different stromal populations and 2 populations of hematopoietic progenitors. Our analysis revealed mesenchymal stromal cells (MSC) as pro-fibrotic cells. MSCs were functionally reprogrammed with loss of their progenitor status and acquisition of a pro-fibrotic phenotype in the fibrotic bone marrow. Additionally, stromal cells exhibited an upregulation of pro-inflammatory mediators like S100A8/A9. We used scRNA 3' RNA sequencing product from 10x Genomics to profile human non-fibrotic bone marrow as well as bone marrow with diagnosed myelofibrosis