Data from: Restriction site associated DNA (RAD) for de novo sequencing and marker discovery in sugarcane borer, Diatraea saccharalis Fab. (Lepidoptera: Crambidae)

We present the development of a genomic library using RADseq (restriction site associated DNA sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer Diatraea saccharalis, an important South American insect pest. A RADtag protocol combined with Illumina paired-end sequencing allowed de novo discovery of 12 811 SNPs and a high-quality assembly of 122.8M paired-end reads from six individuals, representing 40 Gb of sequencing data. Approximately 1.7 Mb of the sugarcane borer genome distributed over 5289 minicontigs were obtained upon assembly of second reads from first reads RADtag loci where at least one SNP was discovered and genotyped. Minicontig lengths ranged from 200 to 611 bp and were used for functional annotation and microsatellite discovery. These markers will be used in future studies to understand gene flow and adaptation to host plants and control tactics.

本研究开发了一套基于限制性酶切位点相关DNA测序（RADseq）的基因组文库构建方案，用于分子标记发掘，可应用于南美重要农业害虫甘蔗螟虫（Diatraea saccharalis）的进化生物学研究。本研究结合RAD标签（RADtag）实验流程与Illumina双端测序技术，从6个供试个体中不仅从头发掘得到12811个单核苷酸多态性（SNPs），还高质量组装得到122.8百万条双端测序读段，总测序数据量达40吉字节。通过对至少携带一个已鉴定并完成基因型分型的SNP的RADtag位点的双端测序读段进行组装，最终获得了分布于5289个微型重叠群（minicontigs）上的、总长约1.7兆碱基的甘蔗螟虫基因组序列。微型重叠群的长度范围为200至611碱基对，后续将用于功能注释与微卫星标记发掘。上述分子标记将在后续研究中用于解析甘蔗螟虫的基因流、寄主植物适应性以及防控策略相关机制。