WIPF1 Regulates Cisplatin-resistant Small Cell Lung Cancer

Small cell lung cancer (SCLC) is a subtype of lung cancer with neuroendocrine characteristics, It is more aggressive, and prone to distant metastasis and resistant to platinum-based drugs, then the prognosis is poor. Cancer stem cells (CSCs) are reported to perform essential role in chemoresistance and recurrence. Increasing clues indicate the significance of WIPF1 in malignant tumor. However, the underlying molecular mechanism has not been fully elucidated in SCLC. Herein, we detect the effects of WIPF1 on colony formation, sphere formation capacity and drug resistance in SCLC cell line model.We expected to find key genes in CSCs maintenance through RNA-seq between enriched CSCs(3D cultures) and differentiated cells. Then using small interfering RNA technology, we conducted WIPF1 knockdown assay. Real -time PCR and western blots confirmed the efficiency of WIPF1-siRNAs. CCK8 assay and colony formation and sphere-formation assay were conducted to observe the role of WIPF1 in SCLC.We found that WIPF1 was up-regulated in sphere-formed cells comparing with differentiated cells under adherent growth conditions in SCLC cell line. WIPF1 was knocked down by WIPF1-siRNA and WIPF1 knockdown could significantly inhibit the capacity of proliferation and enhance the cisplatin sensitivity in small cell lung cancer cells.